Apr 12, 2017 todays promega connections blog is written by guest blogger rachel h. Like the wizard plus sv dna purification system cat. Development and validation of a rapid pcr method for the. Promega manufacturing and delivery systems continue to be fully operational during the covid19 outbreak. To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. A protocol for analysis of mammalian cells containing firefly and renilla luciferase genes, grown in multiwell plates. Information about other promega fluorescent str systems is available upon request from promega or online at. Dualluciferase reporter assay system protocol promega. The powerplex fusion system allows coamplification and fluorescent detection of the core codis us. The sv total rna isolation system provides a fast and simple technique for preparing purified and intact total rna from tissues, cultured cells and white blood cells in as little as one hour, depending on the number of samples to be processed. Id like to have a full download of the whole database or subdatabase. Wsp cld forensic dna continuing education day june 18, 2015. The powerplex 6c matrix standard allow the powerplex fusion 6c system to be analyzed on the applied biosystems 3, 3xl, 3500 and 3500xl genetic analyzers cat. Validation and implementation of the promega powerplex.
The powerplex cs7 allelic ladder mix was analyzed with an applied biosystems 3 genetic analyzer using a 3kv, 5second injection. Thermal cycling time is cut roughly in half, and the system detects substantially more ystr. The powerplex y23 system is designed for use with the geneamp pcr system 9700 with a silver or goldplated silver sample block with max mode as the ramp speed. Promega was an early supplier in the cellfree protein synthesis field and is continuing to develop its portfolio in this area. Str alleles and amplification artifacts pdf free download.
Validation of powerplex y23 system promega using reduced. This standard ensures minimal risk of human dna contamination for products used to collect, store and analyze biological materials for forensic purposes. Shakespeare said, the evil that men do lives after them. Learn how to interpret results from ystr dna typing using the ychromosome haplotype reference database yhrd, a free resource.
Importing powerplex y23 panels, bins and stutter text files into genemapper idx. Validation and implementation of the promega powerplex y23. The powerplex y23 system allows ystr analysis of human forensic and. Features of powerplex y23 system showing the diverse utility of pp y23. Reagent promega in the reaction mix as described in the technical manual. Identification of the remains of king richard iii nature. Either provide a file containing your haplotypes or enter your haplotype manually. It combines common and informative loci to overcome typical challenges faced by laboratories, including sample inhibitors, low levels of input dna, degradation, and mixtures, promega said. Powerplex y23 system is a 23loci, 5color ystr multiplex designed for genotyping of database, paternity and forensic casework samples. Powerplex y23 promega corporation pp y23 system is a 5dye multiplex genotyping kit that analyzes 17 ystr loci, commonly available in other ystr multiplex kits along with that 6 new ystr. Email promega technical services if you have questions on use of this system.
The powerplex esx 17 system is compatible with the abi prism 310, 3100 and 3100avant genetic analyzers and applied biosystems 3, 3xl, 3500 and 3500xl genetic analyzers. The powerplex cs7 system is a multiplex str assay for relationship testing and human identification, allowing coamplification and threecolor detection of seven str loci, including lpl, fb, fesfps, fa01, penta d, penta c and penta e. Jan 30, 2015 promega has released the powerplex fusion 6c system for forensic casework samples. Jul 01, 2015 read highly discriminatory capacity of the powerplex y23 system for the study of isolated populations, forensic science international. Use of the powerplexr y23 system to amplify extracted dna. Promega new powerplexr y23 str system reveals more ystr. This is continually true in the case of unidentified remains throughout the united stat. Yfiler, powerplex y23 promega powerplex y23, yfiler plus applied biosystems. Validation and implementation of the promega powerplex y23 str system marla kaplan dna technical leader. Jul 12, 2012 promega corporation announces the newest addition to the powerplex str systems family, the powerplex y23 systema rapid human identification yshort tandem repeat ystr assay for forensic casework, offender databasing and relationship testing.
Highly discriminatory capacity of the powerplex y23 system. Use of different str kits including powerplex esx 17, powerplex esi 17, and ampflstr ngm, which have different loci adjacent to d3s58, helped verify that the mystery allele was associated with a large d3s58 allele because apparent trialleles changed with each str kit result. A fast, sensitive and reliable ystr multiplex system for forensic and population genetic purpose. Maleline relatives were typed using promega powerplex y23 and for snps defining the main. Our teams are in regular contact with suppliers and distributors worldwide to manage inventory of raw materials to ensure continued availability. These products are intended for preparation of singlesource reference, database and paternity samples where sample purification is unnecessary. Powerplex y23 system reproducibly amplifies trace template amounts as. The powerplex y23 system provides all materials necessary to amplify ystr regions of human genomic dna, including a hotstart dna polymerase, and is compatible with the abi prism 3100 and 3100 avant genetic analyzers and applied biosystems 3, 3 xl, 3500 and 3500xl genetic analyzers. The powerplex y23 system also is compatible with direct amplification, enabling streamlined ystr databasing efforts. By using the speedstar hs polymerase takara bio, otsu, japan in combination with the veriti applied biosystems, foster city. Promega powerplex y23 system receives ndis approval.
Promega corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, highvalue products for the life sciences. The powerplex y23 system is designed for use with the geneamp pcr system 9700 with a silver or goldplated silver sample block. The 5dye str multiplex amplifies 23 ystr loci, including two rapidly mutating ystr loci. The powerplex y23 system is a 23loci, 5color ystr multiplex designed for genotyping forensic casework samples, database samples and paternity samples. A decrease in the amplification time would allow for increased laboratory throughput, which may help reduce backlog when is due to limited cycling capacity. Use of the powerplex r y23 system to amplify dna from.
New york genomeweb news promega said today that its powerplex y23 system has been approved for use in laboratories generating dna records for the national dna index system. Genetics on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Genetic profile of 23 y chromosomal str loci in guizhou shui. The powerplex fusion 6c is a sixcolor, 27locus multiplex system. Developmental validation of the powerplex fusion system for. The file is checked using the validator software and issues, e. Quick 2prtc thermal cycling use of the powerplex y23 system to amplify dna from swabs instructions for use of products dc2305 and dc2320. Strengths and limitations of ngs for forensic dna analysis.
Promega amplification protocol recommends a pcr total reaction volume of 25. The kit contains all 12 loci in powerplex y system, the additional 5 loci found in ampf l str yfiler, plus 6 new loci. The purpose of the present study is to validate the powerplex y23 system using half volume reaction mix for the amplification of a wide range of forensic samples. By using the function search using the export file all haplotypes in the file will be displayed as a list. Navigate to the panels text file downloaded in the getting started section. Maxwell, plexor, powerplex and powerquant are registered trademarks of promega. This manual contains protocols for use of the powerplex fusion 6c system with the geneamp pcr system 9. Powerplex fusion 6c system amplification of 1 ng 2800m dna. However, they were all far from guizhou shui, which suggested that the former three ethnic groups were genetically different from guizhou shui. The powerplex y23 system allows ystr analysis of human forensic and database samples. Richard iii is one of the most famous and controversial english kings. The file needs to be prepared according to the example file found at the bottom of the search page. Esx 17 and esi 17 promega, powerplex fusion promega, powerplex y23 promega.
The sample file was analyzed with the genemapper id software, version 3. Visit the web site to verify that you are using the most current version of this technical manual. The powerplex 16 system is a multiplex str system for use in dna typing, including paternity testing, forensic dna analysis, human identity testing and tissue culture strain identification. The ndis, part of the federal bureau of investigations combined dna index system, contains more than 10 million offender and forensic dna profiles contributed by. A concordance study, forensic science international. Biallelic polymorphism of ag variation at position 308 in. The powerplex fusion system was developed to amplify both extracted dna and dna from solid support materials such as fta cards, swabs, and nonfta punches without compromising performance. The powerplex fusion 6c system provides all materials necessary to amplify str regions of human genomic dna, including a hotstart thermostable dna polymerase, which is a component of the powerplex fusion 6c 5x master mix. Promega forensic products are manufactured in alignment with the iso 18385 standard. The reagent can be added directly to cells in growth medium without washing, and induces cell lysis and acts as a substrate for firefly luciferase, producing a luminescent signal that can be read over a period of two hours. Product components and storage conditions product size cat.
1058 315 1436 342 1411 659 353 19 761 1568 1570 465 323 845 892 1269 550 644 696 1073 410 1311 940 13 989 1357 1573 711 885 55 1381 830 1169 787 1269 1015 509 1295 19 534 993